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Runtogen’s advanced Single B Cell Screening platform enables rapid isolation of native antibody pairs from immunized or convalescent donors. Preserve natural light chain pairing and access the full repertoire of the immune response with high-throughput, single-cell precision.
Unlike display technologies, single B cell screening captures native heavy and light chain pairings directly from primary B cells. This preserves the natural affinity, specificity, and developability of the original immune repertoire.
From immunized animals (or human donors) to lead candidates in as little as 4–6 weeks. Our microengraving and droplet-based platforms screen thousands of B cells per day.
No fusion or cloning steps. Direct RT-PCR from antigen-specific B cells yields antibodies in weeks, not months.
Avoids library bias and artificial combinations. Recover antibodies with natural light chain partners for optimal stability.
Access IgG, IgA, and IgM from memory B cells or plasma cells. Capture rare clones missed by other platforms.
PBMC isolation from immunized hosts (llama, rabbit, mouse, human) followed by B cell enrichment using magnetic beads or FACS.
Fluorescently labeled antigens enable FACS sorting of single B cells binding your target. Sort into 96- or 384-well plates.
Proprietary high-efficiency RT-PCR amplifies native heavy and light chain variable regions from each single B cell.
Bulk cloning into expression vectors, transfection, and rapid small-scale expression for functional screening.
ELISA, SPR, or flow cytometry confirmation of binding, plus biophysical characterization (affinity, specificity).
Advanced droplet microfluidics and FACS strategies enable screening of large B cell repertoires for rare clones.
Validated primers for human, mouse, rabbit, llama, alpaca, chicken, and rat. Custom species available.
Recover complete VH and VL sequences with leader peptides. Directly format as IgG, scFv, Fab, or bispecifics.
Sort by surface markers (CD27, CD138) to target affinity-matured or highly secretory populations.
Soluble proteins, peptides, membrane protein nanoparticles, or transfected cells as sorting baits.
Seamless transition to humanization, affinity maturation, and reformatting for therapeutic leads.
Our optimized single B cell workflow consistently delivers:
Each candidate comes with:
Contact our single B cell screening experts to design a project tailored to your target and species of interest.
Request project consultation →📧 [email protected] | 📞 +1 (303) 522-8502