⚡ CRISPR/Cas9 · Gene Editing Excellence
Knockout Cell Line Service
Custom CRISPR Knockout Cell Lines with Guaranteed Validation
From gene design to validated monoclonal KO cells — delivered in as fast as 4 weeks with over 98% success rate.
✓ CRISPR/Cas9 Editing
✓ Single Clone Validation
✓ Sequencing + WB QC
✓ 4–12 Weeks Delivery
What is a Knockout Cell Line?
A knockout cell line is a genetically engineered model in which a specific gene is permanently inactivated using CRISPR/Cas9 technology. This allows researchers to directly study gene function, validate therapeutic targets, and build reliable disease models. At Runtogen, we go beyond basic gene editing — we deliver fully validated, publication-ready knockout cell lines tailored to your research needs.
🧬 CRISPR/Cas9 Platform
High-efficiency editing with optimized gRNA design and minimal off-target effects.
- ✓ High fidelity Cas9
- ✓ Synthetic sgRNA
- ✓ Custom design
- ✓ Validated guides
01
sgRNA DesignCustom CRISPR guide RNA design for maximum on-target efficiency
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02
CRISPR DeliveryTransfection or viral transduction into target cells
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03
Single‑Cell CloningIsolation and expansion of clonal populations
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04
Validation & QCSanger sequencing, Western blot, functional assays
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05
Final DeliveryKO cells + parental control + full QC report
🔑 Key Applications
🔬 Cancer Research
💊 Drug Target Validation
🧬 Gene Function Studies
📡 Signal Pathway Analysis
🧪 Disease Modeling
🏷️ Biomarker Discovery
⚕️ Rare Disease Research
🧬 Synthetic Biology
📘 Case Study: Human GAL Knockout
Targeted exon 3 of GAL locus in HCT116 cells. CRISPR/Cas9 induced frameshift and complete deletion of 395 bp.
▶ Sanger sequencing: homozygous knockout — del 395 bp (E3 completely deleted)
▸ Wild‑type: CCGAG…GGCAT → Mutant: [395bp deletion] Stop codon
PCR + Western blot confirmed 100% homozygous indel and loss of protein expression.
What is your success rate? +
We achieve a >98% success rate for standard cell lines such as HEK293, HCT116, and A549. For more complex models (e.g., primary cells or iPSCs), our scientists will evaluate feasibility and provide a customized strategy before project initiation.
How do you verify knockout success? +
We use Sanger sequencing and RT-PCR to confirm DNA edits at the target locus, Western blot to validate protein loss, and optional functional assays to confirm phenotypic changes. All raw data is included in the final report.
Can you do double or triple knockouts? +
Yes, dual knockout (double KO) and triple knockout are available upon request. We also offer conditional knockout (Cre-lox) systems for inducible gene inactivation.
What is the turnaround time? +
Typical turnaround is 4–12 weeks depending on gene complexity and cell line. Rush service may be available for urgent projects. We’ll provide a detailed timeline during consultation.
Can you guarantee homozygous knockout clones? +
Yes. We specialize in generating homozygous knockout monoclonal cell lines through optimized CRISPR design and single-cell cloning. All clones are validated by sequencing, and optional protein-level validation (Western blot) is available.
Do you offer guarantees? +
Yes, we guarantee successful generation of your knockout cell line or we will re‑perform the service at no additional cost. Our success rate exceeds 98% for standard projects.
Do you provide custom project design? +
Yes. Our scientists will design a custom CRISPR strategy within 24 hours based on your gene and cell line. This includes gRNA design, feasibility assessment, and timeline estimation.
