Runtogen
Online Inquiry 
1-303-522-8502 
:[email protected]
Online Inquiry 1-303-522-8502 :[email protected]
Runtogen’s MagCRISPR™ KO Cell Line Service is a specialized offering designed to help researchers create cell lines with specific gene knocked out, tailored to their experimental needs. CRISPR Knockout Cell Line service is ideal for studying gene function, disease mechanisms, drug target validation, and more.
CRISPR gene editing is most direct and fastest approach to achieve the knockout of a specific gene or double genes. Runtogen’s CRISPR Gene Knockout Cell Line Service provides end-to-end support for generating custom knockout cell lines using advanced CRISPR/Cas9 gene-editing technologies. Researchers can specify the gene(s) of interest, the cell type, and the level of validation required. Our expert team utilizes CRISPR/Cas9 technology to tailor genome editing to your specific requirements. Whether you require permanent knock-out or conditional knock-out, we have you covered.
CRISPR/Cas9
Synthetic Modified sgRNA (Standard)
Frameshift, stop codon, fragment deletion; Single, Double, or Triple Knockout
Knockout Cell Line Work flow:
Accelerate your research with RuntoGen’s precision-engineered knockout cell lines—contact us today!
Knockout Cell Line Case Study: Human GAL Knockout model in HCT116 Cell Line Service
GAL locus in human colon cancer cell line, HCT116, was knocked-out by creating indels in exon 3, as shown in the targeting strategy.
PCR Identification for positive clones
Individual clones were Sanger sequenced to select for homozygous knock-out indels of GAL at exon 3. Sanger sequencing data. del 251 bp (E3 is completely deleted)
A knockout (KO) cell line is a cell line where a specific gene has been deliberately “knocked out” or deactivated using gene-editing technologies like CRISPR/Cas9. This allows researchers to study the effects of gene loss on cell function, which can be essential for drug development, disease research, and understanding cellular processes.
We create knockout cell lines using advanced CRISPR/Cas9 technology. This technique allows precise editing of the target gene by introducing a double-strand break in the DNA, which is then repaired incorrectly, resulting in gene disruption. We also provide alternative methods such as RNA interference or gene silencing for certain applications.
We work with a wide range of cell types, including tumor cell lines (like HEK293, HeLa, etc.), primary cells, and stem cells. The choice of cell line depends on your research goals and the specific requirements for gene knockout.
The typical timeline for generating a knockout cell line is between 4-8 weeks, depending on the complexity of the project and the cell type being used. This includes the design, transfection, screening, and validation of the knockout.
Our Knockout Cell Line service includes:
*Gene design and CRISPR guide RNA synthesis
*Transfection or viral transduction of cells
*Screening for successful gene knockout
*Clonal isolation and expansion of knockout cells
*Verification via PCR, Western blotting, or other assays
*Custom support for specific needs, including cell culture optimization
We use multiple verification methods to ensure the gene knockout is successful. These may include:Sanger sequencing, Western blot, and functional assays included
Yes, we can create the knockout cell lines for most genes of interest. Our team will first assess the target gene, design appropriate CRISPR guides, and work with you to customize the knockout process based on your specific research goals.
Our success rate for generating functional knockout cell lines is high, typically around 95-100%. Success depends on factors such as gene size, target cell type, and the specific experimental conditions. We will work closely with you to optimize the process and achieve the best possible results.