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Runtogen

Knockout Cell Line Service

Knockout Cell Line Service

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⚡ CRISPR/Cas9 · Gene Editing Excellence

Custom CRISPR Knockout Cell Lines with Guaranteed Validation

From gene design to validated monoclonal KO cells — delivered in as fast as 4 weeks with over 98% success rate.

Request a Quote → Talk to a Scientist →
✓ CRISPR/Cas9 Editing✓ Single Clone Validation✓ Sequencing + WB QC✓ 4–12 Weeks Delivery
98-100%Success Rate
4-12Weeks Turnaround
5,000+Successful Edit

100%Money-back guarantee if no homozygous KO obtained*

What is a Knockout Cell Line?

A knockout cell line is a genetically engineered model in which a specific gene is permanently inactivated using CRISPR/Cas9 technology. This allows researchers to directly study gene function, validate therapeutic targets, and build reliable disease models. At Runtogen, we go beyond basic gene editing — we deliver fully validated, publication-ready knockout cell lines tailored to your research needs.

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CRISPR/Cas9 Platform

High-efficiency editing with optimized gRNA design and minimal off-target effects.

✓ High fidelity Cas9
✓ Synthetic sgRNA
✓ Custom design
✓ Validated guides
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Knockout Type

  • Small Fragment Knockout
  • Frameshift Knockout
  • Large Fragment Knockout
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Available Knockouts

  • Single Knockout
  • Double Knockout
  • Triple Knockout
  • Conditional KO (Cre-lox)
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Cell Line Compatibility

    🔬 Tumor cell lines 🧬 Stem cells
    🧫 iPSC ⚡ Immortalized Cell Lines
 

How Our CRISPR Knockout Service Works

End-to-end support from design to validation

01

sgRNA Design

Custom CRISPR guide RNA design for maximum on-target efficiency

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02

CRISPR Delivery

Transfection or viral transduction into target cells

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03

Single‑Cell Cloning

Isolation and expansion of clonal populations

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04

Validation & QC

Sanger sequencing, Western blot, functional assays

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05

Final Delivery

KO cells + parental control + full QC report

 
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What You Receive

✓ Verified knockout clones (≥2 frozen vials)

✓ Parental control cell line

✓ Full QC report (sequencing + protein validation)

✓ Mycoplasma‑free certificate

✓ 6‑month technical support

 
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Why Choose Runtogen

✓ High CRISPR editing efficiency (>95%)

✓ Multiple clone validation for reliability

✓ Fast turnaround: 4–12 weeks

✓ Experience with 5,000+ KO cell lines

✓ Publication‑ready QC data & free consultation

Key Applications

🔬 Cancer Research 💊 Drug Target Validation 🧬 Gene Function Studies 📡 Signal Pathway Analysis 🧪 Disease Modeling 🏷️ Biomarker Discovery ⚕️ Rare Disease Research 🧬 Synthetic Biology

Case Study: Human GAL Knockout

Targeted exon 3 of GAL locus in HCT116 cells. CRISPR/Cas9 induced frameshift and complete deletion of 395 bp.

GAL Knockout Sanger Sequencing Validation
Figure 1: Sanger sequencing validation of GAL knockout in HCT116 cells
▶ Sanger sequencing: homozygous knockout — del 395 bp (E3 completely deleted)
▸ Wild‑type: CCGAG…GGCAT → Mutant: [395bp deletion] Stop codon

PCR + Western blot confirmed 100% homozygous indel and loss of protein expression.

Comprehensive Validation & Quality Control

Every knockout cell line undergoes rigorous multi‑level validation

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Sanger Sequencing

Confirms DNA editing at the target locus

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RT-PCR

Validate the positive cell pool/clones at mRNA level

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Mycoplasma Testing

Ensures cell line sterility

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Add-On Service

WB, FACS, Whole genome sequencing, etc

Frequently Asked Questions

Everything you need to know about our knockout cell line service

What is your success rate? +
We achieve a >98% success rate for standard cell lines such as HEK293, HCT116, and A549. For more complex models (e.g., primary cells or iPSCs), our scientists will evaluate feasibility and provide a customized strategy before project initiation.
How do you verify knockout success? +
We use Sanger sequencing and RT-PCR to confirm DNA edits at the target locus, Western blot to validate protein loss, and optional functional assays to confirm phenotypic changes. All raw data is included in the final report.
Can you do double or triple knockouts? +
Yes, dual knockout (double KO) and triple knockout are available upon request. We also offer conditional knockout (Cre-lox) systems for inducible gene inactivation.
What is the turnaround time? +
Typical turnaround is 4–12 weeks depending on gene complexity and cell line. Rush service may be available for urgent projects. We’ll provide a detailed timeline during consultation.
Can you guarantee homozygous knockout clones? +
Yes. We specialize in generating homozygous knockout monoclonal cell lines through optimized CRISPR design and single-cell cloning. All clones are validated by sequencing, and optional protein-level validation (Western blot) is available.
Do you offer guarantees? +
Yes, we guarantee successful generation of your knockout cell line or we will re‑perform the service at no additional cost. Our success rate exceeds 98% for standard projects.
Do you provide custom project design?+
Yes. Our scientists will design a custom CRISPR strategy within 24 hours based on your gene and cell line. This includes gRNA design, feasibility assessment, and timeline estimation.

Ready to accelerate your research?

Get a custom CRISPR knockout cell line with validated results and fast delivery.
Our scientists will respond within 24 hours.

Request a Quote → Email Us
✉️ [email protected] 📞 +1 (303) 522-8502

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