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Runtogen

Overexpression Cell Line Service

Overexpression Cell Line Service

Overexpression Cell Line Service

RuntoGen specializes in the development of stable, high-expression cell lines tailored to your research needs. Utilizing advanced lentiviral transduction and PiggyBac transposition technologies, we engineer robust cellular models with consistent and reliable overexpression of your target gene.

 

Our proprietary lentiviral delivery platform ensures high transduction efficiency and stable genomic integration, minimizing variability and enhancing reproducibility. With extensive expertise in gene editing and cell line engineering, we maintain a comprehensive cell repository, accelerating your research by providing ready-to-use, well-characterized overexpression models.

 

 

Key Applications:

 
  • *Gene function studies – Investigate the role of your gene of interest in cellular pathways

  • *Protein interaction analysis – Study overexpression effects on binding partners and signaling cascades

  • *Drug discovery & screening – Validate targets and assess therapeutic responses in engineered cell lines

  • *Disease modeling – Recapitulate pathological conditions by overexpressing disease-associated genes

 

Available Cell Types:

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  • *Tumor cell lines (e.g., HEKs, HeLa, HCT116, A549, etc)

  • *Stem cells (embryonic & adult stem cells)

  • *Induced pluripotent stem cells (iPSCs)

*Primary & immortalized primary cells

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Work Flow and Validation: 

Host Cell Line Evaluation

1-2 weeks

  •  Host cell line will be tested for bacteria, fungi, mycoplasma and other contaminants.
  • Single clone formation efficiency will be tested to determine cell proliferation.

Vector Construction

1-2 Weeks

 

Gene subcloning in a high performing expression vector.

        Lentivirus Packaging

Host cell transfection and generation of stable pools

2-3 Weeks

 

  • Stable transfection
  • Stable Pool selection 

Postive clones screening&Cell expansion

2-3 Weeks

 

  • Characterization of clones
  • QC report

Why Choose Runtogen

Expertise and Precision

With years of experience in gene expression and cell line development, our team brings extensive knowledge and expertise to every project. We use the latest techniques to deliver precise and reliable results.

Reproducibility and Stability

All our overexpression cell lines are tested for long-term stability and reproducibility, ensuring that your results are reliable and consistent throughout your experiments.

Comprehensive Support

From the initial consultation to the final cell line delivery, Runtogen provides expert guidance at every step of the process. Our team is always available to discuss your needs and offer tailored solutions.

Fast Turnaround Times

We understand the importance of time in your research. Our efficient processes ensure rapid generation of high-quality overexpression cell lines, without compromising on accuracy.

Our team at Runtogen is committed to providing high-quality overexpression cell lines that help you achieve your research goals. Contact us now to discuss how our services can accelerate your studies and bring precision to your research.

Case Study: Human TREM2 and Human DAP12 genes were overexpressed in HEK293 cell line

LV-EF1a>hTREM2/EGFP-PGK>Puro

 

Vector Map


LV-EF1a>hDAP12/MyC-DDK-PGK>Puro

 Vector Map


 

Case Study 2: Custom Human HLA-DQB2 Overexpression HeLa Cell Line

 

Vector Map:

 

 

DQB2 melt curve

β-actin melt curve

 

Gene Expression rate (%)

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