Runtogen
Online Inquiry 
1-303-522-8502 
:[email protected]
Online Inquiry 1-303-522-8502 :[email protected]
Tailored Gene Insertion for Advanced Research & Therapeutic Development
Get A QuoteRuntogen’s unique Knockin Cell Line Service enables precise insertion of genetic sequences into specific genomic loci, allowing you to create cell models with endogenous gene modifications that maintain physiological expression patterns.
Using advanced CRISPR/Cas9 and homology-directed repair (HDR) technologies, we deliver knockin cell lines with:
Whether you need reporter knockins, point mutations, or large insertions, our team of genome editing experts will deliver the precise cell models you need for your research.
Industry-leading <1% off-target risk with optimized gRNA design and HDR enhancement strategies.
Support for point mutations, reporter tags, conditional expression systems, and large insertions (up to 10 kb).
Experience with tumor cell lines,Stem cells,IPS cells and challenging primary cells.
Comprehensive genotyping, protein expression, and functional assays included.
As fast as 8–12 weeks from design to validated clones delivery.
Dedicated project managers and scientists to guide you through every step.
Free initial consultation to discuss gene target, insertion type, and cell line. Custom design of gRNAs, donor templates, and HDR enhancers for optimal efficiency.
Synthesis of donor DNA (ssODN, dsDNA, or AAV-based) with homology arms. Optional selection markers (Puromycin, Neomycin) or fluorescence reporters.
CRISPR/Cas9 RNP or plasmid delivery (electroporation, lipofection, or viral methods). HDR enhancement using small molecules (e.g., Rad51 stimulators).
FACS sorting or limiting dilution for monoclonal expansion. PCR genotyping + sequencing to confirm precise knockin.
DNA-level: Junction PCR, Sanger sequencing, NGS (optional). Protein-level: Western blot, flow cytometry, or IF staining. Functional validation: Tailored assays.
2–3 validated clones + isogenic controls. Comprehensive report including protocols and QC data.
| Type | Description | Applications |
|---|---|---|
| Point Mutation KI | Single or multiple nucleotide changes | Disease modeling (e.g., oncogenic SNPs) |
| Reporter Tag KI | GFP, mCherry, NanoLuc, or HA/FLAG tags | Live-cell imaging, protein tracking |
| Conditional KI | loxP-flanked or inducible (Tet-On) systems | Spatiotemporal gene control |
| Large Insertion KI | Gene cassettes (e.g., CAR, antibody genes) | Cell therapy development |
Submit your gene info (NCBI ID, sequence, or donor design) and host cell line name
Receive a customized proposal with timeline and pricing
Project initiation – we handle the rest!
Years of genome editing expertise
Successful KI projects delivered
IP protection guaranteed