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| Description | The NF-κB reporter (luc)-HEK293 cell line is designed for monitoring the nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. |
| Product Name | NFκB-Luc/HEK293 Reporter Cell Line |
| Cat.No | RG-583 |
| Product category | Engineered Cell Lines > Reporter Cell Lines |
| Size | 1*10^6 cells/vial |
| Description | The NF-κB reporter (luc)-HEK293 cell line is designed for monitoring the nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. |
| Species | Homo sapiens, human |
| Full Name | NFκB-Luc/HEK293 Reporter Cell Line, NFκB-Luc/HEK293 |
| Parental Cell Line | HEK293 |
| Biosafety Level | BSL 2. Runtogen determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. |
| Expression Gene | Luciferase |
| Culture Method | MEM+10%FoetalBovineSerum(FBS)+1%NonEssentialAminoAcids(NEAA)+1mMSodiumPyruvate(NaP)+100ug/mlhygromycin |
| Quality Control | NFκB-Luc/HEK293 Reporter Cell Line is negative for mycoplasma, bacteria, yeast, and fungi. |
| Shipping | Cryopreserved cells are shipped with dry ice |
| Storage | Upon arrival, Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Warranty | NFκB-Luc/HEK293 Reporter Cell Line is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product datasheet, website, and COA. |
| Disclaimers | This cell line is intended for laboratory research use only. It is not intended for any human or animal therapeutic use, any animal or human consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from Runtogen. |
If use of this product results in a scientific publication, it should be cited in the publication as: NFκB-Luc/HEK293 Reporter Cell Line (Runtogen RG-583).
Inquire the NFκB-Luc/HEK293 Reporter Cell Line by filling out the form below. We’ll get back to you within 24 hours with a quote.
Inquire the NFκB-Luc/HEK293 Reporter Cell Line by filling out the form below. We’ll get back to you within 24 hours with a quote.
