Knockin Cell Line Generation

Case1 : LIN28B(c.679T>G，p.S227A) (389421) Knockin in A549 Cell Line

Gene knockout strategy:

Sequence：

gRNA1(R):TGCTATAGATGACTTCGTGG AGG

Oligo 1 Sequence:

AGGAGGCTAGGGCAGAGATCTCAGAACGGTCAGGCAGGTCACCTCAAGAAGCTTCCGCACGAAGTCATCTA

TAGCACCAGAAGAGCAAAGCAAAAAGGGGCCTTCAGTTCAAAAAAGGA

PCR Result:

Sanger Sequencing: